qPCR is widely used in gene expression analysis, it also can be used for copy umber determination. In qPCR, there is a relation between the PCR cycle at which the fluorescent signal crosses a defined threshold (Ct) and the initial amount of input DNA (copy number). By comparing Ct value between the target locus and reference locus, a delta Ct value can be generated and used for CNV calculated.
Technique Highlights
– Low screening cost
– Fast turnaround time
– Short assay development time
– Hight sensitivity
– Open format (not restricted to a single supplier)
Sample Requirement
Sample form: Genomic DNA
Sample Quantity: >= 5 ug
Sample Conc.: >= 100 ng/ul
Sample Purity: OD260/280 = 1.7~2.0
Sample without severe degradation
Service Workflow
1. Project strategy consultation and custom experimental design
2. Sample receiving
3. DNA extraction and QC
4. qPCR
5. Project final report
Deliverables
1. Copy number analysis result
2. QC result
