SNPseq is a Genesky propriety high-throughput SNP genotyping multiplex system. SNPseq utilises the highly specific ligation reactions to capture a large number of targeted genomic fragments containing the SNPs of interest. After PCR amplification using universal primers with sequences designed at one end of the ligation probe, all the PCR products are sequenced on NGS sequencer. All SNPs genotypes can be reported by bioinformatics analysis on NGS data.
Technique Highlights
– High Throughput 1000s of SNPs can be genotyped in one reaction, and 1000s of samples can be genotyped
together using multiplex sequencing. With one Illumina HiSeq2500 sequencer, over 8 million SNPs can be genotyped
within 3 days, whereas only one or several samples can be handled at a time with traditional microarray-based
approaches.
– Cost-Effective Compared to other commercially available SNP microarrays, all probes and reagents in Genesky
propriety SNPseq assay are carefully designed or selected with affordability in mind.
– Simplified Hands-on Workflow One ligation reaction, one PCR amplification, one purification, that’s all the steps
needed before sample uploading on the NGS sequencer, avoiding often tedious and labor-intensive hands-on
operations with traditional microarray-based approaches.
– High Accuracy Thanks to the high-specificity of ligation reaction and since unspecific ligation products can be
effectively excluded during NGS data analysis, the genotyping accuracy of SNPseq are substantially increased and
stands out among other SNP genotyping platforms.
Sample Requirement
Sample Type: Genomic DNA
Sample Quantity: DNA >= 5 ug; concentration >= 100 ng/ul
Sample Purity: OD260/280 = 1.7~2.0
Sample without severe degradation
Service Workflow
1. Cusomtized experimental design
2. Sample receiving
3. DNA extraction and QC
4. SNPseq multiplex genotyping
5. Project final report
Deliverables
1. SNP genotyping analysis result
2. QC result
