Genesky proprietary iMLDR is an improved ligase-based multiplex SNP genotyping system. Compared to traditional ligase-based assays, both accuracy and pass rate are substantially improved with iMLDR. Preliminary data from multiple replication studies and double-blind tests demonstrated the accuracy of iMLDR is consistently higher than 98%. The performance of iMLDR is superior than most of the currently available genotyping platforms in terms of both accuracy and cost-effectiveness.
Technique Highlights
– Cost-effective Double-ligation allows the repeated use of the same set of pre-synthesized fluorescent probes for
allele discrimination, dramatically reducing the cost spent on the synthesis of sequence-specific fluorescent probes in
traditional ligase-based assays
– High accuracy Accuracy of SNP calling constantly above 98%
– Medium Throughput Most appropriate for 15-30 SNPs multiplex genotyping
Sample Requirement
Sample Type: Genomic DNA
Amount: DNA >= 5 ug; concentration >= 100 ng/ul
Purity: OD260/280 = 1.7~2.0
Sample without severe degradation
Service Workflow
1. Cusomtized sequencing experimental design
2. Sample receiving
3. DNA extraction and QC
4. iMLDR multiplex genotyping
5. Project final report
Deliverables
1. SNP genotyping analysis result
2. QC result
